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Platforms & Hosts
Microbial systems are fast, scalable and economical — if you pick the right host and engineer the process to match. Our toolbox spans bacterial and yeast hosts, three expression modes and a full analytical stack, tied together by our FoldForge development platform.
01 — Expression systems
Our highest-throughput host for recombinant proteins, peptides, scaffolds, antibody fragments and plasmid DNA. Multiple strain backgrounds and promoter systems let us tune toward titer, solubility or secretion.
A eukaryotic secretion workhorse for disulfide-rich and glyco-amenable proteins. High cell-density fermentation and a clean secreted product simplify capture and reduce downstream cost.
A GRAS, naturally secreting host ideal for industrial enzymes and larger secreted proteins. Low endotoxin and simple, chemically defined media keep purification lean.
For difficult targets we deploy additional yeast and bacterial systems, including chaperone-augmented and protease-deficient strains, to express novel molecular formats others can't.
02 — Process types
Where your protein ends up — the medium, the periplasm or an inclusion body — defines the entire downstream. We develop all three and choose by data.
Product is exported to the medium or periplasm, easing capture and reducing impurity load. Preferred for correctly folded, disulfide-containing proteins in yeast and Bacillus.
Active, folded protein accumulated in the cytoplasm. We balance induction, temperature and chaperones to maximize soluble yield and avoid aggregation.
Very high expression captured as dense inclusion bodies, then solubilized and refolded. Our refold screening platform recovers active product from the toughest constructs.
03 — Proprietary platform
Our integrated strain-and-process development engine. FoldForge pairs miniaturized parallel fermentation with automated downstream and analytics to compress months of empirical work into structured, predictive design cycles.
Each program runs through a closed loop: construct libraries are screened in micro-bioreactors, the best clones advance into DoE-driven fed-batch, and a calibrated scale-down model de-risks the jump to commercial volumes before a single GMP litre is made.
parallel micro-bioreactors per development cycle
from construct to feasibility titer read-out
scale-down to at-scale titer correlation
refold conditions screened per difficult target
04 — Analytical capabilities
| Category | Methods | Applied to |
|---|---|---|
| Identity & purity | SDS-PAGE, CE-SDS, RP/SEC/IEX-HPLC, peptide mapping, intact mass | Release & characterization |
| Higher-order structure | CD, DSC, FTIR, analytical ultracentrifugation | Comparability, folding QC |
| Process impurities | Host-cell protein ELISA, residual DNA qPCR, endotoxin (LAL) | Clearance & safety |
| Potency & bioassay | Cell-based and enzymatic activity, binding assays | Lot release |
| Stability | ICH forced-degradation & real-time/accelerated programs | Shelf-life claims |
Match the molecule
Send us a sequence and a target profile. We'll come back with a recommended host, expression mode and a feasibility plan.